Plant glutathione transferases (GSTs) have a major role in plant herbicide detoxification system. PvGSTU2-2 has been isolated from leaves of Phaseolus vulgaris plants. The enzyme catalyses the conjugation of glutathione with chloroacetamide herbicides alachlor and metalachlor. In order to study in planta the ability of PvGSTU2-2 to detoxify chloroacetamide herbicides, we developed transgenic tobacco plants overexpressing PvGSTU2-2. The introgression of the transgene in the plant genome was confirmed by RT-PCR and the expression of PvGSTU2-2 with q-RTPCR analysis. In addition, it was verified that the transgenes codes functional proteins by measuring GST enzyme activity towards NBD-CI in transgenic lines. Three PvGSTU2-2 overexpressing lines were assayed for their tolerance towards the herbicide dimethenamid (chloroacetamide). Under in vivo conditions, T0 lines exhibited increased tolerance at 0,5 and 1 mg/L dimethenamid, with significantly increased shoot and root elongation compared to wild type plants. These results confirm that overexpression of PvGSTU2-2 in tobacco provides a way of conferring selectivity and enhancing crop safety and production.
Isoenzyme, Tobacco plants, Common beans, Glutathione transferases, GSTs
Type
Text (Conference or workshop item (Poster))
Project Notes
Πράξη: ΘΑΛΗΣ-ΓΕΩΠΟΝΙΚΟ ΠΑΝΕΠΙΣΤΗΜΙΟ ΑΘΗΝΩΝ- Μεταφοράσες γλουταθειόνης (GSTs): μοριακά εργαλεία για ανάπτυξη βασικής και εφαρμοσμένης έρευνας στα πεδία της πράσινης και κόκκινης βιοτεχνολογίας
Υποέργο: Μεταφοράσες γλουταθειόνης (GSTs): μοριακά εργαλεία για ανάπτυξη βασικής και εφαρμοσμένης έρευνας στα πεδία της πράσινης και κόκκινης βιοτεχνολογίας
Μελέτη: Ερευνητικά άρθρα και παρουσιάσεις σε συνέδρια των αποτελεσμάτων της Πράξης